The SFO alleged Peter Johnson, Jonathan Mathew and Stylianos Contogoulas had conspired to defraud between 1 June 2005 and 31 August 2007.
The Libor investigation was launched in 2012 and Barclays has since been fined £290m for rigging rates.
The trio will appear at Westminter Magistrates’ Court next week. The SFO continues its investigation.